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KMID : 0363020050350020277
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 2 p.277 ~ p.288
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Inhibition of MMP-13 mRNA expression by ginseng saponin in fetal rat calvarial cells
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김양이
최득철/김영준
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Abstract
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There is a potential role of collagenase-3 in alveolar bone loss and periodontal disease
progression, we need to develope or find chemotherapeutic drugs or herbal agents which
may regulate the expression of MMP-13. Ginseng saponin, one of the major components of
Korea ginseng(panax ginseng) root, has many various biologic effects, such as cytotoxic
effect, tumoricidal effects, cytokine regulations, and protein biosynthesis effect. The purpose
of this study was to determine the effects of Korea red ginseng saponin on MMP-13 gene
expression in osteoblasts.
The experimental groups were cultured with ginseng saponin in concentration of 1.0, 10,
25, 50, 100, 250 and 500㎍/㎖ for MTT assay. Primary rat calvarial cells were pre-treated for
1 hour with ginseng saponin(100 ㎍/㎖) and then stimulated with IL-1β(1.0ng/㎖) and PTH
(10 nM). MMP-13 gene expression was evaluated by RT-PCR.
The results were as follows:
Ginseng saponin was cytotoxic to osteoblast at concentration exceeding 250㎍/㎖ for longer
than 24 hours in tissue culture(p<0.01). In RT-PCR analysis, steady state MMP-13 mRNA
levels were increased approximately 350% by IL-1β, and 400% by PTH when normalized to
untreated control. IL-1β-indued MMP-13 mRNA expression was reduced 50% by pretreatment with ginseng saponin. But ginseng saponin didn't inhibit MMP-13 expression from
PTH stimulated cells.
This results suggest that ginseng saponin inhibit IL-1β-indued MMP-13 mRNA expression.
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KEYWORD
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ginseng saponin, MMP-13, RT-PCR, primary rat calvarial cell
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